Uploading data

To upload your raw data to Flow, first navigate to the "Upload data" page under "Actions" in the leftmost menu.

Here, you are able to upload demultiplexed fastq files, multiplexed fastq files and other data types.

Demultiplexed fastq files

Simply select whether your sample is paired or single-end and click in the dashed box to choose your files.

Once you've chosen your files you are able to add your metadata. It is most important to give your sample a Name, choose the Category of sample (ie. the method used like RNA-Seq) and select the Organism your sample was generated from. For now Flow supports six species (human, mouse, rat, drosophila, yeast and zebrafish), and soon you will be able to add your own. For certain sample categories other metadata is required, for example 5' barcode sequences are always required for CLIP-Seq samples.

Multiplexed fastq files

Sometimes your data might have complex barcoding that is not handled by your sequencing facility, therefore we provide the option to upload multiplexed data that can be demultiplexed on Flow.

For multiplexed data, annotation is provided as an excel spreadsheet, with a single spreadsheet per sample category. For example, if your multiplexed fastq contains both CLIP-Seq and RNA-Seq you would provide one annotation spreadsheet for the CLIP-Seq samples and one for the RNA-Seq samples. The concept of a sequencing "lane" refers to one multiplexed fastq file and all of its associated annotation. Here you are able to create a new lane, or add files to an existing lane.